Effect of Surfactant Inclusions on the Yield and Characteristics of Protease from Bacillus Subtilis
نویسندگان
چکیده
In this study, the effect of surfactants (Tween-80 and Acetonitrile) inclusions in the culture medium of Bacillus subtilis on the yield and characteristics of extracellular protease was investigated. The results showed that addition of Tween-80 increased the enzyme yield by 5 folds and thermal stability from 50C to 60C. Tween-80 did not affect the optimum pH, KM and VMax values as well as the enzyme efficiency. Acetonitrile shifts the optimum pH from 8 to 7 and reduced the affinity of the enzyme for casein. The selection of appropriate surfactants for Bacillus subtilis culture is required for optimum protease production.
منابع مشابه
Biological Control of African Violets Root-Knot Disease by the Used of Extracellular Protease Bacillus
The present study explored the efficacy of Bacillus spp. and protease production for biocontrol of the root-knot nematode Meloidogyne javanica in African violet media. Among 100 bacterial isolates from various soils, the highest nematode mortality was observed for treatments with isolate GM-18, which was identified as Bacillus subtilis based on cultural and morphological characteristics and 16S...
متن کاملIsolation, Purification and Characterization of a Thermophilic Alkaline Protease from Bacillus subtilis BP-36
The goal of this research was to isolate and identify the thermostable alkaline protease producing bacteria among several native Iranian microorganisms. At the end of screening program, a Bacillus subtilis BP-36 strain producing thermophilic alkaline protease was isolated from a hot spring in Ardebil province. The target enzyme was purified using a one-step Aqueous two-phase systems (ATPS) prot...
متن کاملCloning and Enhanced Expression of an Extracellular Alkaline Protease from a Soil Isolate of Bacillus clausii in Bacillus subtilis
in the detergent industry. In this study, the extracellular alkaline serine protease gene, aprE, from Bacillusclausii was amplified by PCR and further cloned and expressed in B. subtilis WB600 using the pWB980 expression vector. Protease activity of the recombinant B. subtilis WB600 harboring the plasmid pWB980/aprEreached up to 1020 U/ml, approximately 3-folds higher than the nativ...
متن کاملMOLECULAR CLONING AND EVALUATION OF WILD PROMOTER IN EXPRESSION OF BACILLUS SPHAERICUS PHENYLALANINE DEHYDROGENASE GENE IN BACILLUS SUBTILIS CELLS
To evaluate the role of wild promoter of L-phenylalanine dehydrogenase (PheDH) gene, referred to as pdh, from Bacillus sphaericus in expression, cloning of pdh gene in Bacillus subtilis was performed. The whole pdh gene was cloned in pHY300PLK shuttle vector and amplified, construct (pHYDH) then transformed in B. subtilis ISW1214 and E. coli JM109. The pdh endogenous promoter presented no effec...
متن کاملInvestigation of the effect of biosurfactant of Bacillus subtilis against Staphylococcus strains biofilms
Background: Biosurfactants are compounds that are produced by different microorganisms and have an emulsifying property. This study aimed to investigate extractive biosurfactant from bacillus subtilis (PTCC1720) against the biofilms of Staphylococcus aureus (PTCC 1112), Staphylococcus saprophyticus (PTCC 1440) and Staphylococcus epidermidis (PTCC 1435). Materials and Methods: This study was con...
متن کامل